Use of controls or performance of procedures other than those specified in the user's manual or adjustments to the specified procedures may result in hazardous radiation exposure.
The rear panel of the QuantumXpert™ clearly displays:
The startup procedure entails startup of the QuantumXpert™'s laser and warm-up of the QuantumXpert™'s detectors.
The QuantumXpert™ comes equipped with a dual channel 491nm + 532nm laser with an average power output of 20mW at each wavelength. The following startup procedure may be performed before or after startup of the FCSXpert™ software:
If the laser interlock becomes disconnected, the laser controller will indicate an error (the red LED labeled 'ERROR' will light up) and will not illuminate until it is reset. Follow these steps to reset the QuantumXpert™'s laser:
If after performing the laser reset procedure the red 'ERROR' led is still on, please contact technical support for assistance.
The QuantumXpert™ uses Nunc™ Lab-Tek™ 8-well sample chambers (Model #: 155409).
Samples can be loaded using one of the following two methods:
Samples should be pipetted into the chambers so that the sample covers the bottom of the well and so that the height of the liquid is > 1 mm. These requirements are usually satisfied with minimum volumes of 200-250 µL, depending on the surface tension of the solvent.
In cases where minimal sample consumption is desired, 10 µL droplets can be used in place of the standard 200 µL volume. Simply pipette the 10 µL drop into the center a well of the chamber.
The measurement aperture of the QuantumXpert™ is located under the sample cover on the side nearest the right edge of the instrument, so the sample chamber should be inserted so that the well to be measured is facing the outer edge of the QuantumXpert, as shown to the right.
The sample chamber may be loaded with or without the chamber cover, and may also be sealed to prevent evaporation.
The sample chamber can be moved into four different position for measuring each of the four wells that are facing the outer edge of the QuantumXpert. The positions are indicated on the sample guide.
When the first position is reached, the sample drawer will click into place and the first line on the sample guide will be aligned with the line on the sample drawer.
Each well position can be reached by sliding the sample drawer until the respective line on the sample guide is aligned with the line on the sample drawer.
To unload the sample chamber, simply slide the sample drawer until the entire sample chamber is visible and slide the sample chamber out from the sample drawer.
The sample chamber may be discarded or saved for measurement again at a later time.
The shutdown procedure only entails shutdown of the QuantumXpert™'s laser. The QuantumXpert™ itself can remain powered and ready for use by leaving +5V and +12V power supplies connected.
The following shutdown procedure may be performed before or after shutdown of the FCSXpert™ software: